ABL's scientific staff can develop the assays our clients require to determine the immunogenicity and efficacy of their products in animal models or from human samples. Our scientists are highly experienced in optimizing both novel and traditional assays specific to the needs of our clients’ research and product development pipelines. We have a wealth of experience doing this work in mouse and non-human primate (NHP) models and have continued to expand our experience working with human samples.

We partner with clients to help determine the needs of their immunomonitoring or testing programs, and develop assays that will provide data in a rapid and efficient manner.

These are just a sampling the assays we have developed and optimized for client specific studies:

Cellular Immunity

• Immunophenotyping
• CD3, CD4, CD8, CD45, CXCR4, CXCR5 from PBMC’s, lymph nodes, GALT, tissues, mucosal washes
• ELISPOT – mouse and rhesus
• Intracellular cytokine staining
• Luminex
• FACS analysis to quantitate or determine production of IFN-gamma, TNF-alpha, IL-2, IL-4, IL-5, IL-6, IL-10
• Memory ICC staining
• Cytometric bead array
• Flex array system to target other cytokines/chemokines
• Lymphoproliferation assay

Humoral Immunity

• Serological Assays
• ELISA – serum and mucosal
• Functional and neutralizing antibodies
• PBMC and target cell-based assays
• RIPA, Western blot, and IFA
• Cell surface antibody binding by FACS

Molecular Assays

• Real Time PCR

 

For analytical development in support of therapeutic and vaccine release.  Please click here.